Knowledge Center

Poster / Apr 25, 2017

On the use of Differential Scanning Fluorimetry to Assess the Impact of Spray Drying on Superoxide Dismutase for Dry Powder Inhaler Formulations

Authors:
  • Diana Fernandes
Source:
RDD Europe 2017
  • When spray drying a biopharmaceutical(BP), its integrity should be maintained upon processing.
  • The integrity of a biopharmaceutical can be determined by monitoring its conformational stability through its melting Temperature (Tm) shift.
  • Differential Scanning Calorimetry (DSC) and Dynamic Light Scattering (DLS) are two analytical techniques usually employed to determine Tm

However,

  1. these require large sample volumes at high concentrations
  2. are time-consuming

Differential Scanning Fluorimetry might be an alternative

  • allows simultaneous screening of more than one sample;
  • at low concentration requirements.

Convenient when dealing with expensive molecules such as biopharmaceuticals!